Gain Insight into Human Oocyte Surface Morphology with Tescan CLARA SEM

Studying the surface morphology of human oocytes is essential for understanding the process of fertilization. However, light microscopy imaging techniques lack the resolution and contrast required for detailed oocyte surface analysis.

Due to complex topography , dedicated DEPTH mode was used to capture fine structural details of the egg-sperm interaction to completely visualize all details with high depth of focus.

Using both in-chamber and in-beam secondary electron (SE) detectors provided a complementary imaging advantage that is especially important for delicate, complex samples like human oocytes.

Visualize oocyte surface morphology and gamete interaction with clarity and structural integrity using Tescan SEM Systems.

Visualizing Human Oocyte Surface Morphology

Spare human gametes, donated from IVF treatments, were chemically fixed and stained, dehydrated, coated with gold, and prepared for high-resolution SEM imaging.

High-resolution imaging was performed using the Tescan CLARA UHR-SEM under high vacuum conditions. Due to the topographically complex surface of oocytes, a dedicated DEPTH mode was employed to maintain sharp focus across the entire surface.

Images were captured using the In-chamber secondary electron (SE) detector, the In-beam SE detector, or a combination of both  . The in-chamber detector emphasizes overall surface topography, while the in-beam detector provides high-resolution detail at short working distances. By combining signals from both detectors, we obtained comprehensive images with high resolution and accurate contrast.

This approach enabled detailed visualization of sperm–oocyte interactions, including multiple sperm heads and acrosomal remnants, vesicle structures containing enzymes critical for oocyte penetration.

High-Resolution Imaging of Oocyte Surface Morphology and Sperm Interaction

Ultra-high resolution SEM revealed the complex surface of human oocytes and detailed sperm-oocyte interactions, including acrosomal remnants critical for fertilization.

Using both in-chamber and in-beam SE detectors provided simultaneous topographic context and fine structural detail, preserving delicate structures while delivering ultra-high resolution, high-contrast images.

This approach reveals ultrastructural details far beyond the reach of light microscopy, enabling detailed analysis of gamete interactions, and unlocking new potential for both basic research and reproductive medicine.