Achieve high-quality cryo-ET data through precise, contamination-free sample preparation. Tescan AMBER G cryogenic FIB-SEM workflows enable targeted thinning of vitrified specimens while maintaining native biological architecture, ensuring optimal lamella quality for downstream tomography.
Dedicated cryo-stages, integrated transfer systems, and controlled vacuum environments protect fragile samples from devitrification and ice contamination. Automated FIB milling and stage stability deliver reproducible lamella thickness and site-specific targeting for consistent cryo-TEM results.
Generate structurally intact, reproducible cryo-lamellae that reveal organelle organization, protein complexes, and native cell ultrastructure with confidence.
Preserve Native Biological Structure for Reliable Cryo-ET and Volume Electron Microscopy
Achieving reproducible cryo-ET data starts with precise sample preparation and preserved structural integrity. Tescan AMBER G delivers cryogenic FIB-SEM workflows that maintain native cell and tissue morphology. This enables confident cryo-lamella preparation, high-resolution surface imaging, and 3D volume reconstruction in life-science research.
FOR THE ENTIRE LIFE SCIENCES RESEARCH AND DEVELOPMENT PROCESS
Cryo ET Sample Preparation
.webp?width=1264&height=1020&name=2_A%20correlation%20of%20%20SEM%20(1).webp)
Surface Morphology Analysis
Characterize biological surfaces with clarity and precision. Tescan AMBER G delivers high-resolution cryogenic FIB-SEM imaging that preserves native morphology without dehydration or coating. This reveals true ultrastructural detail at the cell and tissue level.
Variable-pressure and low-kV imaging modes minimize beam damage, while advanced detectors enhance topographical contrast for hydrated or beam-sensitive samples. Controlled cryo-conditions and automation ensure repeatable, artifact-free surface imaging across diverse life-science specimens.
Gain reproducible morphological insights into membranes, organelles, and tissue interfaces to support accurate biological interpretation.
Volume Electron Microscopy
Reconstruct biological structures in 3D with nanometer precision. TESCAN AMBER G combines automated FIB milling with high-resolution SEM imaging to generate detailed volumetric datasets of cells, tissues, and organelles.
Stable cryogenic conditions and precise beam control preserve ultrastructure, while automated slice-and-view imaging ensures consistent alignment and uniform resolution across large volumes. Integrated data processing enables accurate 3D reconstruction and correlative light-electron analysis.
Reveal subcellular organization and spatial relationships that deepen biological understanding.
"Tescan AMBER X 2, our latest innovation in materials analysis technology. The AMBER X 2, with its advanced Plasma FIB-SEM capabilities, offers unmatched speed, precision, and versatility, setting a new standard in the field."
PETR KLIMEK
Product Marketing Director Material & Geo Science
Tescan
Used in This Workflow
Tescan AMBER 2
TESCAN AMBER 2 is a cryogenic Ga FIB-SEM workstation for Life Sciences research. This enables precise cryo-lamella preparation, surface morphology analysis, and 3D volume electron microscopy under stable cryo conditions.
Its field-free UHR SEM column and integrated cryo-stage preserve native cellular architecture and deliver reproducible nanoscale imaging across biological samples.
- Cryogenic FIB-SEM: site-specific thinning of vitrified cells and tissues for cryo-ET and cryo-TEM
- Structural preservation: controlled cryo-environment prevents devitrification and ice contamination during milling and imaging
- Integrated workflows: automated slice-and-view and correlative light-electron imaging for reliable 3D reconstruction
