FIB-SEM tomography combines a focused ion beam with ultra-high resolution SEM to deliver the best Z-resolution. This method employs a FIB to sequentially mill nanometer-thin layers and SEM to image each exposed surface, enabling the capture of complex biological structures with exceptional precision. It reveals subcellular architecture and spatial relationships that are not visible in a single imaging plane. Moreover, plasma FIB is ideal for processing hard biological materials, such as bones or shells, enabling detailed 3D reconstructions across diverse specimen types.
Look Beyond the Surface
Understanding biological systems requires more than a single plane of imaging—how organelles interact within cells, how cells organize in tissues, or how microbial communities form in ecological niches. Tescan volume electron microscopy (vEM) provides multi-scale, 3D insights into cells, tissues, and small organisms.
FOR VOLUME ELECTRON MICROSCOPY
FIB-SEM TOMOGRAPHY
SERIAL BLOCK-FACE IMAGING
Serial Block-Face SEM (SBF-SEM) is ideally suited for high-resolution 3D imaging of cells, tissues, and small organisms. By repeatedly removing thin slices with a fully integrated ultramicrotome and imaging each freshly exposed surface, it captures detailed data across the entire volume, preserving the full context of the sample. This technique is perfect for studying structural relationships across extended regions, mapping cellular networks, and analyzing rare events. By combining high-contrast imaging with efficient volume acquisition, SBF-SEM provides reliable, detailed 3D reconstructions for a wide range of biological applications.
ARRAY TOMOGRAPHY
Array Tomography combines serial ultrathin sectioning with automated SEM imaging to generate high-resolution 3D reconstructions of cells, tissues, and small organisms. Sections are cut using a standalone ultramicrotome, collected on a ribbon, and automatically imaged using the dedicated AT software module. Large overview images and optional correlation with fluorescence data allow precise targeting of regions of interest, reducing unnecessary acquisition time. The Dedicated Array Tomography software module guides the user through slicing and imaging setup. This enables reliable volumetric reconstruction while preserving sections for re-imaging or further analysis.
for EVERYDAY ELECTRON MICROSCOPY WORKFLOW
Tescan CLARA UHR-SEM
Tescan CLARA is engineered for advanced biological imaging under both ambient and cryogenic conditions. It supports a wide range of workflows, enabling researchers to capture complex biological structures and visualize them in 2D and 3D with exceptional detail.
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BrightBeam™technology delivers ultra-high resolution and sharp, contrasted images even at very low beam energies.
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In-Flight™ beam tracing and automated alignments enable users to optimize imaging and analysis quickly and consistently.
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Wide Field Optics™ allows distortion-free navigation from 1× overview down to nanoscale features
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MultiVac™ Imaging Mode automates low-vacuum aperture and water-vapor operation for imaging of sensitive samples.
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TESCAN Essence™ interface ensures effortless operation and multi-user support for streamlined operation across all experience levels.
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ConnectomX Katana integrated ultramicrotome
Tescan SOLARIS X2
Tescan SOLARIS X2 combines MISTRAL™ Xe plasma FIB with UHR-SEM immersion optics for fast and precise milling and high-quality imaging. Its enhanced beam profile enables fast milling and low-energy polishing, ideal for large-volume and high-throughput workflows.
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MISTRAL™ Xe Plasma FIB Column: Enhanced beam profile for fast milling and precise low-energy polishing.
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UHR-SEM Immersion Optics: Optimized for high-quality imaging.
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Tescan Essence™ Software: Designed for multi-user environments, ensuring effortless operation regardless of experience level.
SOLARIS X2 delivers stable performance in both ambient and cryo conditions. With intuitive navigation, it ensures consistent, reproducible results across even the most demanding applications.
Tescan AMBER X2
High-performance Xe plasma FIB-SEM system designed for demanding cryo and room temperature structural analysis.
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3D FIB-SEM tomography of large volumes and/or hard materials such as shells, bones, and dental tissue
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MISTRAL™ Xe Plasma FIB Column: Enhanced beam profile for fast milling and precise low-energy polishing.
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Field-free UHR-SEM optics ideal for multipurpose labs
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Tescan Essence™ Software: Designed for multi-user environments, ensuring effortless operation regardless of experience level.
Tescan AMBER X2 combines the speed and efficiency of plasma FIB milling to enable high-throughput structural analysis under both ambient and cryogenic conditions. It delivers reliable, artefact-free results for advanced life science research, whether it's large-volume 3D tomography of hard biological materials or high-quality cryo lamella preparation.
Tescan SOLARIS 2
Advanced analytical tool for 2D and 3D characterization of your biological and beam-sensitive samples. It combines Ga FIB with immersion optics.
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Low-keV, high-contrast BSE imaging – Reveal resin-embedded biological structures in 2D and 3D with exceptional clarity.
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UHR-SEM Immersion Optics – Provides detailed imaging for challenging biological samples, ensuring accurate observation of delicate ultrastructures.
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Best resolution Ga+ FIB column (< 2.5 nm resolution) with optimized ion optics for excellent performance and reproducibility
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Tescan Essence™ Software: Designed for multi-user environments, ensuring effortless operation regardless of experience level.
Designed for precision and flexibility, SOLARIS 2 brings together low-keV, high-contrast imaging with advanced analytical and correlative workflow to clearly visualize biological ultrastructure in 2D and 3D.
Tescan AMBER 2
Flexible and robust solution designed to meet the complex requirements of modern research centers.
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UHR BrightBeam™ optics deliver high-contrast imaging of non-conductive and beam-sensitive samples at very low kV.
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Best resolution Ga+ FIB column (< 2.5 nm resolution) with optimized ion optics for excellent performance and reproducibility
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FIB-SEM tomography: Ultra-high resolution 3D reconstructions of cells and cellular structures
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Seamless switching between ambient and cryogenic modes
Tescan AMBER 2 provides life scientists with a comprehensive platform for both cryogenic and ambient applications. From UHR imaging of beam-sensitive samples to fully integrated correlative cryo workflows, it preserves biological integrity while delivering high-resolution insights into tissues, cells and subcellular architectures.
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